Ithuluzi leChromatogarphy

Isihlungi sesirinji se-PVDF sinezinzuzo eziningi. Ifilimu ye-PVDF inamandla amakhulu emishini, akulula ukuphuka noma ukugoqa; inezinzuzo zesizinda esiphansi, ukuhambisana okubanzi kwe-solvent, nekhono elihle kakhulu lokufaka imibala; ngaphezu kwalokho, ifilimu ye-PVDF ingahlolwa ngokuphindaphindiwe ngemva kokwelashwa okufanele. Usayizi wembotshana we-PVDF ulwelwesi ngu-0.22¦Ìm no-0.45¦Ìm. I-0.22¦Ìm ifanele amaprotheni anesisindo samangqamuzana esingaphansi kuka-20kDa, anganciphisa ngempumelelo ukuvuza kwamaprotheni amancane esisindo samangqamuzana. Uma kuqhathaniswa nolwelwesi lwe-PVDF lwe-0.45¦Ìm, inenani eliphakeme le-protein adsorption kanye nesivuno sokulandelana kwamaprotheni. Isibonelo, umthamo we-adsorption we-insulin ne-BSA izikhathi ezingu-1.64 kanye nezikhathi ezingu-1.58 kulwelwesi oluvame ukusetshenziswa lwe-0.45¦Ìm PVDF, ngokulandelana.

I-septa ka-Aijiren yokuvala ngezikulufo ezingu-24mm isebenzisa i-Silicone yombala wemvelo kanye ne-PTFE. Lawa ma-Septa anobubanzi obungu-22mm kanye nobukhulu obungu-3mm, ahambisana nobukhulu be-Cap futhi angabekwa phakathi kwe-Vial ne-Cap ukuze kuhlukaniswe umoya nesampula. Usayizi 3mm. Ugqinsi lubuye lufaneleke kakhulu ukubhoboza inaliti. Izinto ze-Septa zithambile futhi ziqinile, azikhubazeki kalula futhi ngeke zephule.

U-Aijiren uncoma ukuthi isisefo sesirinji ngasinye sisetshenziswe cishe izikhathi eziyi-10. Uma kukhona ukungcola okuningi kakhulu ku-reagent, usayizi wembotshana wesihlungi sesirinji uzovinjelwa futhi umsebenzi wokuhlunga awukwazi ukuqedwa. Isihlungi sesirinji esinikezwe i-Aijiren sinenani elincomekayo kanye nekhwalithi enhle. Kufaneleke kakhulu ukushintshela kusihlungi esisha Sesirinji ukuze uqhubeke nokuhlunga ngemva kokusisebenzisa izikhathi ezimbalwa.

I-Aijiren ikhiqize i-24mm Screw Neck Closures iminyaka engaphezu kwengu-20. Ukusuka ekucutshungulweni kwe-Cap kuya ekukhiqizweni kwe-Septa, konke kukhiqizwa ngemishini ezenzakalelayo. U-Aijiren uthembisa ukusebenzisa ama-workshops angenalo uthuli ukukhiqiza i-Screw Closures ukuqinisekisa ukuhlanzeka kanye nezinga eliphezulu lemikhiqizo.

Umkhiqizo odumile ka-Aijiren, i-Headspace Vial, unezinhlobo ezimbili: I-Screw Neck ne-Crimp Neck. Umsebenzi osesithombeni upakisha i-Vial ye-headspace ngemva kokukhiqizwa kwayo. Ngemva kokulebula umkhiqizo, ukupakishwa kwangaphandle kuyadingeka. Lokhu ukupakisha okunezendlalelo eziningi. Ingakwazi ukumunca ukushaqeka ukuze ivikele i-Headspace Vial ukuthi ingalimali ngenxa yamabhampa ngesikhathi sokuthutha.

Isikulufu esingu-9mm esikhiqizwe i-Aijiren sinembobo emaphakathi ukuze kube lula ukuthathwa kwesampuli yenaliti yomjovo ye-autosampler. Inaliti yomjovo izongena ku-septa futhi ingene ngaphakathi kwe-vial ukuze ikhishwe. Iphethini yomucu ye-screw cap engu-9mm ilingana eduze ne-vial. I-Septa icindezelwa kancane phakathi kwekepisi ne-vial ukuze kugcwaliswe igebe, ngakho-ke ukuvala kuphezulu kakhulu.

I-COD Test Tube ekhiqizwe i-Aijiren ivamise ukusetshenziselwa ukuhlaziya izinga lamanzi, ukuqapha indawo ezungezile, ukutholwa kwesidingo komoyampilo kanye nokunye ukuhlola kwamakhemikhali. Lokhu kudinga izinto ezingavuthiwe eziphezulu zokukhiqizwa kwe-COD Test Tube, futhi kudinga ingilazi ezinzile nengasebenziyo ukuze kukhiqizwe. Izidingo zobuchwepheshe zabakhiqizi ziphakeme kakhulu. Njengefekthri enamashumi eminyaka esipiliyoni sokukhiqiza, i-Aijiren ingawuphatha kalula lo msebenzi.

Ikepisi lebhodlela le-PP lekhwalithi ephezulu eliluhlaza okwesibhakabhaka le-9mm Screw Neck Closures lizohlanganiswa ne-Septa. I-Aijiren inendlela eyingqayizivele enelungelo lobunikazi yokuhlanganisa i-Silicone ne-PTFE. I-Septa ekhiqizwa ngale ndlela enelungelo lobunikazi ihlanganiswa ne-Cap engasebenzi ngamakhemikhali ukuze kugwenywe ukuhwamuka okukhulu futhi kugcinwe ibhodlela lesampula livalwe kahle. Kunconywa ukugcinwa kwesikhashana noma i-chromatography ewuketshezi. I-Aijiren ikhiqiza imibala eyahlukene ye-9mm Screw Neck Closures ongakhetha kuyo ukuze uhlangabezane nezidingo ezahlukene.

Ukuvalwa kwe-Aijiren kuvame ukuba yi-100pcs njengephakethe, kanti i-Headspace Vial ehambisanayo nayo i-100pcs njengebhokisi. Lolu hlobo lokupakisha lulungele ukusetshenziswa ngamakhasimende. I-Aijiren inganikeza isethi ye-Via and Closures ethengwe ndawonye, ​​futhi iphinde isekele ukuthengwa Kokuvalwa ngokuhlukana.

Abasebenzi base-Aijiren esithombeni bapakisha i-Micro-Insert. Lolu hlobo lwe-Micro-Insert ngokuvamile lufanele i-Autosampler Vial. Ku-Autosampler Vial engu-9mm, 10mm, 11mm caliber, umthamo ungama-300ul, kanti ububanzi obungaphandle bungu-5 noma 6mm micro-insert. Zombili zingashintshwa, kodwa ezitsheni ze-Autosampler ezinobubanzi obungu-8mm, kungasetshenziswa kuphela ama-micro-insert ane-diameter yangaphandle engu-5mm.

I-septa ekhiqizwe i-Aijiren isebenzisa izinto ezingavuthiwe zekhwalithi ephezulu. Izinto ezingavuthiwe ziyathambisa futhi zishiswe zibe yisikhunta sibe yisimo esinosayizi nosayizi ofanayo. Khona-ke, inqubo eyingqayizivele isetshenziselwa ukuhlanganisa i-Silicone ne-PTFE, futhi ekugcineni isikwe futhi isikwe usayizi ofanele i-Vial. . I-Aijiren, njengemboni yerabha, inolwazi olwanele namandla okuqinisekisa ikhwalithi ye-Septa.

U-Aijiren ungumkhiqizi ochwepheshe onguchwepheshe ekukhiqizeni Izitsha ze-Autosampler, Izitsha ze-Headspace, Ibhodlela le-Reagent, i-Syringe Fiter, njll. Indlela yokukhiqiza eyamukelwa i-Aijiren iwukukhiqiza okuzenzakalelayo okuzenzakalelayo, okuqinisekisa ukuthi yonke i-vias ihlolwa ngokuqinile futhi ihambisana ne-ISO standard. Isithombe sibonisa ishabhu yokupakisha yase-Aijiren. I-Vias ekhiqiziwe izopakishwa emabhokisini futhi ilinde ukuthunyelwa endaweni yokusebenzela yokupakisha.

I-Aijiren ithola isihlungi sosayizi esifanele ukuze kuqinisekiswe ukuthi amakhasimende angakwazi ukuhlunga izinhlayiya ngendlela efanele. Kunconywa ukuthi ukhethe ububanzi besihlungi ngokucophelela. I-Aijiren ivame ukukhiqiza osayizi abangu-13 mm no-25 mm. Ngamunye wabo unekhono elihlukile lokubamba izinhlayiya. Ngokuya ngosayizi wesampula, ububanzi bokuhlunga obufanele bungakhethwa. Isibonelo, ububanzi obuyi-13 mm bungasetshenziswa kumasampula we-1-10 ml. Ngokufanayo, izihlungi zesirinji ze-PVDF ezingama-25 mm zingasetshenziselwa isampula yevolumu engu-10-100 ml. Ukugcinwa kwabo kungaphansi kwe-100 ml.

I-screw cap engu-18mm isebenziseka kalula futhi ayidingi amathuluzi engeziwe, njengekhepha nesivula. Amasampula angadluliselwa esizeni, asikho isidingo sokudlulisa amasampula elabhorethri. Uchungechunge olunembayo luqinisekisa ukucinana komoya phakathi kwe-Headspace Vial kanye nesikulufa sikazibuthe esingu-18mm. I-Magnetic nut ingasetshenziswa kabanzi ekuhlaziyeni okuhlukahlukene kwe-SPME kanye ne-headspace.<\/p>

I-9mm Screw Neck Closures-Polypropylene Screw Cap enezingqimba ezimbili ze-Septa ehlanganisiwe inganikeza uphawu oluvimbela ukuhwamuka. I-double layer ihlanganisa i-Silicone ne-PTFE. Le Septa ehlanganisiwe ingasetshenziselwa imijovo eminingi. Futhi ifaniswe ne-PTFE elayishiwe isikulufu cap yamasampula ezinto eziphilayo. I-screw cap ihlinzeka nge-inert, i-air-tight seal kanye nokusetshenziswa kwenaliti yesirinji ukuze kungene isampula ngokuqondile.

Ukuhambisana noma ukumelana kwamakhemikhali kwe-20mm Grey Butyl Septa namasampula nezincibilikisi kungabonwa umthengisi we-Aijiren. Ukuqina kwe-Butyl kwenza i-20mm Grey Butyl Septa ilungele imijovo eminingi noma isitoreji. I-20mm Grey Butyl Septa inogqinsi oluyi-3mm, enezici zokuvala ezinhle futhi yenza kube lula ukubhoboza inaliti ye-autosampler. Ayijiyile ngokwanele ukuthi ingathinta ukubhoboza. I-PTFE ukumelana kakhulu kwe-septum, futhi inqubo yokubopha i-Aijiren yenza i-Septa ingabi lula ukuwa.

Izitsha zesampula ezijwayelekile ze-GC kanye ne-HPLC zinokuvuleka okungu-9mm ukuze kube lula ukugcinwa kwesampula. Ingasetshenziswa ngokuvala okungu-9mm. Kunemibala ehlukene yekhava evulekile, ikhava eqinile kanye nezinhlobonhlobo ze-septa ezifanele izinhlelo zokusebenza ezahlukene ukuze amakhasimende azikhethe. U-Aijiren ukhiqiza Ukuvala ekamelweni elihlanzekile. Kuyimizamo ka-Aijiren yokuqinisekisa izinga eliphezulu le-9mm Closures kanye ne-9mm hplc vial.

I-Aijiren's 9mm Screw Neck Autosampler Vial ingenye yemikhiqizo edume kakhulu ye-Aijiren. I-screw neck kanye nomthamo ongu-2ml kuhambisana kakhulu nokucaciswa kwe-autosampler, ikakhulukazi i-Aijiren's 100pcs\/pack, elula kakhulu ukuthi amakhasimende ayisebenzise. Yamukelwe kahle, inozinzo oluqinile futhi ayithinteki kalula ngamanye ama-reagents.

I-Crimp Neck Closures ekhiqizwa i-Aijiren ivame ukusetshenziswa ku-Crimp top Headspace Vial. Ukukhiqizwa kweCrimp Neck Closures kuzenzakalela ngokugcwele. Njengemboni eneminyaka engu-20 yokuhlangenwe nakho kokukhiqiza, i-Aijiren ingahlangabezana nekhwalithi ye-Crimp Neck Closures futhi ibe umkhiqizi owaziwayo wezinto ezisetshenziswayo ze-chromatographic e-China.

U-Aijiren ubambe iqhaza ekukhiqizeni izinto ezisetshenziswayo ze-chromatographic iminyaka engama-20. Izinhlobo zemikhiqizo ekhiqizwe i-Aijiren zifaka i-Autosampler Vial, Isihlungi Sesirinji, Ishubhu Yokuhlola I-COD, I-Headspace Vial, Ibhodlela Le-Reagent, njll. Le mikhiqizo idinga ukupakishwa futhi ithunyelwe ngemva kokukhiqizwa kwe-Aijiren. Lokhu kusebenzisa ishabhu yokupakisha esesithombeni.

Indlela yokukhetha isihlungi sesirinji esinembayo: Izihlungi zesirinji ezilahlwayo ze-Aijiren ziza ngezinhlobo eziningi. Kukhona ikakhulukazi uchungechunge olubili, olulodwa luyi-hydrophilic kanti olunye luwuchungechunge lwe-hydrophobic. Isihlungi sesirinji sinobubanzi obubili: 13mm no-25mm. Usayizi wembotshana wolwelwesi lwesihlungi ngu-0.22, 0.45um. Izinto ze-Membrane zifaka: I-RC, inayiloni, i-PVDF, i-PTFE, njll. Kubalulekile kumakhasimende ukukhetha ulwelwesi olulungile.

Intambo yekepisi yensimbi kazibuthe ilingana eduze ne-Headspace Vial, futhi ubukhulu be-Septa bungaqinisekisa futhi ukuthi iyaminyana kancane ukuze kuzuzwe umphumela wokuvala lapho ikepisi livalwa yiVial. I-Cap enembobo emaphakathi ingasiza umjovo wesampula ngaphandle kwama-reagents avuzayo. Umsebenzi we-magnetism ukulingana kangcono ne-autosampler.

I-screw cap engu-18mm isebenziseka kalula futhi ayidingi amathuluzi engeziwe, njenge-capper nesivula. Amasampula angadluliselwa esizeni, asikho isidingo sokudlulisa amasampula elabhorethri. Uchungechunge olunembayo luqinisekisa ukucinana komoya phakathi kwe-Headspace Vial kanye nesikulufa sikazibuthe esingu-18mm. I-Magnetic nut ingasetshenziswa kabanzi ku-SPME ehlukahlukene kanye nokuhlaziywa kwe-headspace.

U-Aijiren ungumhlinzeki ochwepheshe wezinto ezisetshenziswayo ze-chromatography. I-Aijiren ayihlinzeki nje ngokuvala okuphezulu kwe-Crimp engu-20mm, kodwa futhi inikeza i-Headspace Vial efanayo, i-Hand Crimper kanye ne-Decrimper. I-20mm Crimp Top Closures inomsebenzi oqinile wokuvala, futhi inhlanganisela yerabha ne-PTFE yenza Ukuvala kube nokusebenza kangcono kokuvala futhi kulungele kakhulu ukubhoboza inaliti yomjovo ngokuzenzakalelayo. I-Aijiren inolwazi lweminyaka engu-20 ekukhiqizeni izinto ezisetshenziswayo ze-gas chromatography. Imikhiqizo yekhwalithi ephezulu kanye nesistimu yokubuyekeza eqinile yenze i-Aijiren yaphumelela kuze kube manje.

Ngaphezu kwalokho, impahla ye-Butyl inquma ukuthi i-Septa akulula ukuyilimaza, noma isetshenziswe isikhathi eside, iyanwetshwa. I-Aijiren isebenzisa izinto zokusetshenziswa zekhwalithi ephezulu, eziqinisekisa ukuthi i-Butyl Septa ngeke iphuke futhi ingcolise isampula uma ingene ngomjovo ozenzakalelayo ngesikhathi.

I-butyl ka-Aijiren kanye ne-PTFE eyinhlanganisela ye-Septa ivamise ukusetshenziswa ku-20mm Aluminium Crimp Cap. Le nhlanganisela ye-Closures ifaneleka ku-20mm crimp headspace vials. Ugqinsi oluyi-3mm lwe-Septa lulungele ukuvalwa futhi ngeke lonakaliswe ukubhoboza. Ikhanda lesampula. Njengendali yefekthri eqondile, i-Aijiren ikulondolozela izindleko zokuthengisa eziqoqiwe zabasabalalisi bezinga eliphakathi. Intengo inokuncintisana kakhulu futhi izinzuzo zayo zobuchwepheshe zinikeza ukuqinisekiswa kwekhwalithi okuthembekile kweqoqo ngalinye lemikhiqizo ethunyelwa ngaphandle kwemboni, ukuze amakhasimende akwazi ukuyisebenzisa ngokuqiniseka.

I-Septa ka-Aijiren eyenziwe ngezinto ezingavuthiwe zekhwalithi ephezulu ingavikela uphawu ngaphakathi kwe-Vial ngezinga elithile, futhi ngeke iphuke noma iwe ikepisi ngemva kokubhobozwa inaliti yomjovo ozenzakalelayo. Lokhu ngokwezinga elikhulu. Ukuhlolwa kwe-gas chromatography kunikeza ukunethezeka, futhi kuletha imiphumela ephephile nenembile.

Irack yesampula enezikhundla ezingu-50 inezimbobo eziyindilinga futhi ubude obuseceleni bakhelwe amabhodlela e-headspace. Uhlaka oluqinile lubunjwe kusuka kumazinga okushisa aphezulu kanye ne-high-pressure polypropylene. Ikhwalithi ephezulu-the...

Isihlungi sesirinji se-Aijiren¡¯s sinezinhlobo eziningi ezihlukene zamalwelwesi. I-Aijiren ingaphinda ithengise ulwelwesi ngokwehlukana. Ama-reagents ahlukene adinga ukukhetha ulwelwesi lwezinto ezihlukene ukuze agweme ukusabela phakathi kwe-membrane nama-reagents futhi athinte imiphumela yokuhlola. I-Aijiren ivamise ukuncoma amakhasimende ukuthi akhethe i-PTFE, ngoba i-PTFE ayisebenzi ngokwedlulele futhi ifanele ama-reagents amaningi.