20ml headspace vial33mm Isihluzi sesirinji sithengiswa

Izinga lokubophelela kwiprotheyini ephantsi: Ezi zihluzo zinezinga eliphantsi lokubophelela kwiprotheyini. Xa isampulu ibotshelelwe kancinci, ukwanda kwayo kunyuka, kwaye ikwaneempawu ezingcono zokusasaza. Iisampulu ezihlanjululwe ngeefilitha ze-PES zinokuvelisa iziphumo ezingcono ngokunyamezela utshintsho oluncinci kwiipropathi zabo ezinxulumene nokuxinana.

Inayiloni: Izihluzi ze-nayiloni zemembrane zine-hydrophilic, zibhetyebhetye, azikwazi ukukrazula, kwaye ziyakwazi ukuzenzela. Ziyakwazi ukumelana noluhlu lwe-solvents eziphilayo kwaye zilungele ukusetyenziswa kunye neesampuli eziphezulu ze-pH. Inayiloni ibopha iiprotheni. Ayizifanelanga izisombululo ezineasidi. Uluhlu lwe-pH ~ 3-14.
Esi sihluzi se-hydrophilic MCE sesirinji esilahlwayo sibonelela ngokucoca okusebenzayo kwiisampulu ezinamanzi. Umgangatho onzulu wezindlu we-PP wonyango uqinisekisa ukuhamba okucocekileyo kunye nokutywinwa okuthembekileyo, ngelixa i-premium ye-MCE membrane igcina ukusebenza ngokucokisekileyo kokusetyenziswa kwebhubhoratri yesiqhelo.

Ngokusebenzisa izihluzi ze-Aijiren zesirinji amasuntswana angafunekiyo ayasuswa anokuthi ngenye indlela abangele ungcoliseko kunye nokuvala izixhobo ezibuthathaka. Ke, ukuhluza iisampulu zakho kukhokelela ekonyukeni kobomi kunye nexesha elingaphantsi leekholamu zechromatographic kunye nezixhobo, kunika iziphumo ezingaguquguqukiyo kunye neziphindaphindekayo kwaye ngoko konga ixesha kunye nemali.

Kule nqaku, siza kujongana ne-20 ebuzwa rhoqo malunga ne-0.45 ye-syringe filters kwaye sinikeze iimpendulo ezibanzi ukukunceda wenze izigqibo ezinolwazi malunga neemfuno zakho zokucoca.Hlunga isampuli yakho ngaphambi kwesixhobo se-chromatography, inokukunceda isixhobo sakho se-chromatography. I-Aijiren lab membrane yesihluzi sesirinji lolona khetho lulungileyo.

Isihluzo sesirinji sisixhobo esincinci, esilahlwayo esiqhele ukusetyenziswa kwizicwangciso zelabhoratri ukuhluza okanye ukucacisa ulwelo. Yenzelwe ukuba ifakwe ekupheleni kwesirinji kwaye isebenze njengesithintelo esisusa amaqhekeza kunye nokungcola kumanzi njengoko kunyanzeliswa ngesihlunu.

Ngenxa ye-hydrophobicity ephezulu ye-membrane ye-PVDF, kufuneka ifakwe kwi-ethanol, i-methanol okanye ezinye izisombululo ezifanelekileyo zokuqalisa. Le mveliso kuphela kuphando lwezenzululwazi ngamagcisa, kwaye kufuneka ingasetyenziselwa ukuxilongwa kwekliniki okanye unyango, okanye isetyenziselwa ukutya okanye amayeza, kwaye akufanele igcinwe kwiindawo zokuhlala eziqhelekileyo. Ukhuseleko kunye nempilo yakho, nceda unxibe iidyasi zaselebhu kunye neeglavu ezilahlwayo xa usebenza.

Isihluzo se-membrane ye-MCE sibonelela abaphandi ngelona liphantsi lidityanisiweyo lokucoca. Ngenxa yeempawu zabo ezibophelelayo eziphantsi kakhulu, ezi zihluzo zibonelela ngemveliso ephezulu kuneemveliso ezikhuphisanayo kwaye zinciphisa ukutshintshwa kwesihluzo xa kuhluzwa izisombululo zeprotheyini.

I-membrane ye-MCE inikezela ngezakhono ezikhethekileyo zokugcina, iinqununu zokubambisa kunye ne-microorganisms ezincinci njenge-0.22 μm ngobukhulu. Olu hluzo lulungileyo lunceda ukukhusela izixhobo zocazululo ezinovakalelo, iinkqubo ze-HPLC, kunye nezinye izixhobo zaselebhu ekungcolisekeni, zigcina ukusebenza kwazo ngeyona ndlela kunye nokwandisa ubomi bazo.

Izihluzi ze-PTFE zesirinji ziquka i-polytetrafluoroethylene (PTFE) inwebu kunye ne-polypropylene engaphezulu kwendlu. Ifumaneka kwi-13 mm kunye ne-25 mm ububanzi kunye ne-0.22 μm kunye ne-0.45 μm ubukhulu bepore. I-PTFE yinwebu ekhethiweyo yokusetyenziswa kunye nezinyibilikisi ezinobundlobongela, ulwelo, kunye neegesi ezinokuhlasela ezinye iinwebu./p>

Izihluzi zesirinji ze-Aijiren luludwe olubanzi lwezihluzi zesirinji ezilahlwayo ezingahlambulukanga ukulungiselela ulungiselelo oluthembekileyo lwesampulu. Umgangatho we-membrane oveliswayo kunye neenkqubo zokuvelisa ezizenzekelayo ziqinisekisa...
Izihluzi zesirinji zePVDF zenziwe nge-hydrophilic PVDF inwebu kunye ne-polypropylene overmold housing. Ifumaneka kwi-13 mm kunye ne-25 mm ububanzi kunye ne-0.22 μm kunye ne-0.45 μm ubukhulu bepore.

Umahluko okhethekileyo wombala onikezelwe kumphezulu wefilimu ye-MCE unceda ukufumanisa amasuntswana kunye nokunciphisa ubunzima bamehlo. Kukulungele ukubuyiswa kunye nokugcinwa kweebhaktheriya ekubaleni ikholoni kunye novavanyo lwe-sterility, kunye nohlalutyo lwamanzi //amanzi amdaka kunye nolunye uvavanyo lwe-microbiological.

Sebenzisa i-Aijiren CA i-syringe filter ukususa izinto eziphazamisayo kunye neencinci ezincinci kwi-pore elinganisiweyo yobungakanani, ngaloo ndlela ufumane isicatshulwa sesampuli esicocekileyo. Izihluzi zeCellulose acetate (CA) zesirinji zilungele ukuhluza imidiya yenkcubeko yethishu kunye nokucoca iisampulu zebhayoloji ezinovakalelo. Ukongeza inyathelo lokuhluza isirinji phambi kwesitofu sechromatographic akuncedi kuphela ukuqinisekisa iziphumo ezihambelanayo nezithembekileyo, kukwanceda ukukhusela isixhobo kunye nokwandisa ubomi bekholamu. Ukhetho loqoqosho Izihluzi zesirinji ziyafumaneka kwiindidi ezahlukeneyo zobukhulu kunye neentlobo ze-membrane ukuhlangabezana neemfuno zaso nasiphi na isicelo.

Umahluko okhethekileyo wombala onikezelwe kumphezulu wefilimu ye-MCE unceda ukufumanisa amasuntswana kunye nokunciphisa ubunzima bamehlo. Kukulungele ukubuyiswa kunye nokugcinwa kweebhaktheriya ekubaleni ikholoni kunye novavanyo lwe-sterility, kunye nohlalutyo lwamanzi //amanzi amdaka kunye nolunye uvavanyo lwe-microbiological.

Izihluzi zesirinji zikholisa ukuba nendlu yeplastiki enesihluzo senwebu ngaphakathi. Indlu inesitshixo se-luer wesifazane okanye uqhagamshelo lwe-luer slip oluvumela ukuba luncanyathiselwe ngokukhuselekileyo kwisirinji. I-membrane filter, eyona nto ibalulekileyo, yenziwe ngesixhobo esinemingxuma esivumela ulwelo ukuba ludlule ngelixa igcina amasuntswana kunye nezinto ezingcolisayo ngaphezu kobukhulu obuthile.

Izihluzi ze-PTFE zesirinji ziquka i-polytetrafluoroethylene (PTFE) inwebu kunye ne-polypropylene engaphezulu kwendlu. Ifumaneka kwi-13 mm kunye ne-25 mm ububanzi kunye ne-0.22 μm kunye ne-0.45 μm ubukhulu bepore. I-PTFE yinwebu ekhethiweyo yokusetyenziswa kunye nezinyibilikisi ezinobundlobongela, ulwelo, kunye neegesi ezinokuhlasela ezinye iinwebu.

13mm, 25mm 0.22um, 0.45um hydrophobic PVDF (polyvinylidene fluoride) inwebu yesihluzi sesirinji sisixhobo sokuhluza esiqhele ukusetyenziswa kwizicwangciso zebhubhoratri ukususa amasuntswana kunye neemicroorganisms kulwelo.

Isihluzi seSiringe seLab seMCE sisixhobo sokuhluza esiguquguqukayo esilungiselelwe izicelo zaselabhoratri. Yakhiwe ngekhwalithi ephezulu edibeneyo ye-cellulose ester (MCE) imathiriyeli, ezi zihluzo zesirinji zibonelela ngokugqwesa ukuhambelana kweekhemikhali kunye nokuqina. Basusa ngokufanelekileyo ii-particle, microorganisms, kunye nokungcola kwiisampuli zolwelo, ziqinisekisa ukucoceka kwesampulu eyongeziweyo kuhlalutyo oluchanekileyo.

I-Polypropylene iyahambelana ne-acetone, i-ethyl acetate, i-methanol, i-isobutyl alcohol, i-methanol, kunye ne-methyl ethyl ketone. I-Polypropylene ayihambelani ne-cyclohexane, i-ethers, i-dichlorobenzene, i-pentanes, i-methylene chloride, kunye ne-trichlorobenzene. Igesi, i-disinfectants okanye i-autoclave iyacetyiswa ukuba inzalo yeemveliso ezenziwe nge-polypropylene. Ubushushu obuninzi bokusetyenziswa yi-135¡ãC.

Ukuhluza inzala, oko kukuthi, ukususa iintsholongwane ngeendlela eziphathekayo ezifana nokuhlola okanye ukucoca imathiriyeli yokucoca, yindlela eqhelekileyo esetyenziswayo yokuvala inzala. Indlela yokucoca ibanzi...

Injongo yesihluzo sesirinji kukususa ubumdaka, amasuntswana, okanye izinto ezingafunwayo kwisampulu engamanzi phambi kokuba itofwe okanye ihlalutywe. Isihluzi se-membrane ngaphakathi kwesihluzo sesirinji sisebenza njengesithintelo, ngokukhetha sivumela kuphela amasuntswana athile okanye iimolekyuli ukuba zidlule ngelixa sigcina amasuntswana amakhulu.

Nge-PES isirinji ye-membrane yokucoca i-membrane eyomeleleyo ngoomatshini, inokumelana nolwelo olunobushushu obuphezulu, ngesantya esikhulu sokuqukuqela (ngcono kune-PTFE), kunye nokubophelela kweprotheyini ephantsi. Ngezi mpawu, izihluzi ze-PES zesirinji zibonelela ngokusebenza okuphezulu ukuze zisetyenziswe kunye neesampulu zebhayoloji kunye nenkcubeko yemidiya. Uluhlu lwe-pH olusebenzayo ~ 3-14.

Isihluzi sesirinji se-Aijiren sePVDF sine-hydrophilicity kunye nokuhambelana kakuhle kweekhemikhali. Banokuzisa ukuchaneka kuphando lwakho kwaye baqinisekise umgangatho ophezulu wokusebenza. Uluhlu lwethu lokucoca luyilwe nge-leur-lock inlet yabasetyhini kunye ne-Leur-slip outlet yendoda. Ukongeza, ezi zihluzo zinokuchasana ngcono koomatshini kwaye zinokumelana namaqondo obushushu aphezulu.

Iifilitha ze-Aijiren's Syringe zikumgangatho ophezulu, zipakishwe kakuhle kwaye zithengiswa ngexabiso elifanelekileyo lokukhuphisana. Zifumaneka kwizinto ezininzi eziphambili zembrane ezibandakanya iNayiloni, i-PTFE, i-PES, i-MCE, i-PVDF, i-CA, i-PP, kunye ne-GF ebonelelwa ngeefomathi ze-13mm, 17mm, 25mm kunye ne-30mm kwii-virgin polypropylene housings.

Isihluzo sesirinji sisixhobo sokucoca esikhawulezayo, esiluncedo nesithembekileyo esisetyenziswa rhoqo kwilabhoratri.Kuba akufuneki ukuba batshintshe inwebu kunye nokucoca ukucoca, kuphelisa ulungiselelo olunzima kunye nexesha. Isetyenziswa ngokubanzi kwisampulu yokucoca kwangaphambili, ukususwa kwamasuntswana, ukuhluzwa kwe-aseptic yolwelo kunye neegesi. Yeyona ndlela ikhethwayo yokucoca iisampulu ezincinci phambi kokulayisha kwi-HPLC kunye ne-GC, kwaye ihlala isetyenziswa kunye nesirinji elahlwayo.

Iifilitha ze-0.45 zesirinji ziqhele ukusetyenziswa kuphando kunye nezicwangciso zebhubhoratri kwiinkqubo ezahlukeneyo zokuhluza. Baziwa ngokukwazi kwabo ukususa amasuntswana kunye ne-microorganisms kwizisombululo, okwenza zibe sisixhobo esibalulekileyo kwiinkalo ezininzi zesayensi. Nangona kunjalo, ngokhetho oluninzi kunye neengqwalasela ekufuneka zithathelwe ingqalelo xa ukhetha isihluzo sesirinji, kuyinto eqhelekileyo ukuba nemibuzo kunye neenkxalabo.